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Бр. на производ:
90643777
Chaps Lysis Buffer - Each (50ml)
Бр. на производ:
90643777
MKD 5589
Price Details
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Manufactured in an ISO 9001 and 13485 certified facility in the USA, this buffer is essential for cell lysis and protein extraction.
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What Stands Out
Optimized Formulation
Chaps Lysis Buffer offers a carefully optimized formulation to ensure efficient cell lysis, helping researchers maximize protein yield and integrity for various applications.
Versatile Application
This buffer is suitable for a wide range of biological samples, making it ideal for diverse research needs in proteomics, diagnostics, and cellular studies.
Convenient Packaging
The 50ml volume ensures sufficient supply for multiple experiments, providing convenience and reducing waste for laboratories looking to streamline their workflow.
Детали за производот
- Manufactured in an ISO 9001 and 13485 certified facility in the USA
- For research use only. Not for human or animal clinical/therapeutic use.
- Each production lot is manufactured in our USA laboratory following stringent Good Manufacturing Practices and rigorous quality control tests under certified 14644 clean room conditions
- This buffer is used for cell lysis and protein extraction.1x Concentration Contains:50mM Tris-HCl (pH 7.4)110mM NaCl5mM EDTA1% CHAPS.CHAPS is 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate Warning:For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.
- For cell lysis and protein extraction
| Container Type | Bottle |
| Item Form | Liquid |
| Item Weight | 0.25 Pounds (110 grams) |
| Volume | 50 Milliliters |
| Brand Name | eCon Lab Supply |
| Manufacturer | Ciro Mfg |
| Part Number | 23.105.120 |
| Included Components | Bottle |
| Brand | eCon-Lab-Supply |
| Item Volume | 50 Milliliters |
| Package Information | Bottle |
What's in the box
Bottle
Who Should Buy?
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Molecular Biologists
Ideal for researchers requiring efficient cell lysis and extraction of proteins or nucleic acids for analysis.
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Laboratory Technicians
Facilitates smooth protocols in laboratory settings, ensuring consistent and reliable performance across various samples.
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Academic Researchers
Supports studies in cellular biology with effective lysis of different organism cells, enhancing experimental outcomes.
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Non-Scientific Users
Unsuitable for individuals without laboratory experience as safe handling and knowledge of applications are required.
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Casual Experimenters
Not designed for home use or casual experimentation where basic tools and protocols may be lacking.
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Field Researchers
Field conditions often lack the controlled environment necessary for using this buffer effectively without degradation.
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Прашања и одговори на купувачи
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Прашање:
What is Chaps Lysis Buffer used for?
Одговор: Chaps Lysis Buffer is primarily used for cell lysis in various biochemical applications. It effectively disrupts cellular membranes, allowing for the extraction of proteins and nucleic acids. This is crucial for researchers who require purified cellular components for analysis, such as enzyme assays or protein characterization. For instance, scientists studying membrane proteins often opt for Chaps due to its mild surfactant properties, which preserve the integrity of these fragile molecules during extraction. -
Прашање:
How do I prepare Chaps Lysis Buffer?
Одговор: To prepare Chaps Lysis Buffer, you typically need to dissolve Chaps in a suitable buffer solution, often Tris or phosphate buffer, and adjust the pH as necessary. It’s essential to ensure that all components are mixed thoroughly to achieve the desired concentration and effectiveness. Using freshly prepared buffer is recommended, particularly for sensitive applications like protein analysis. For example, lab technicians often prepare this buffer just before experiments to maintain maximum efficiency in protein extraction processes. -
Прашање:
Is Chaps Lysis Buffer suitable for all cell types?
Одговор: While Chaps Lysis Buffer is versatile, its effectiveness may vary depending on the cell type. It is particularly suitable for mammalian and yeast cells, but less effective for certain plant cells or bacterial cells where different lysis buffers might be more appropriate. Researchers often conduct preliminary tests to determine the optimal lysis buffer for their specific cell type, ensuring efficient extraction of cellular components for their downstream applications. -
Прашање:
What are the advantages of using Chaps Lysis Buffer?
Одговор: Chaps Lysis Buffer offers several advantages, including its mild nature, which helps maintain protein functionality during lysis. Unlike harsher detergents, it minimizes protein denaturation, making it ideal for applications where protein activity preservation is crucial. Additionally, its amphipathic properties allow for effective solubilization of membrane proteins, providing researchers with high-quality samples for detailed analyses. This buffer is commonly used in studies involving ionic interactions and membrane dynamics. -
Прашање:
Can Chaps Lysis Buffer be used for protein purification?
Одговор: Yes, Chaps Lysis Buffer can be effectively used for protein purification. Its ability to solubilize proteins while maintaining their structures allows researchers to separate target proteins from cellular debris efficiently. This is especially useful in techniques like affinity chromatography and gel filtration, where maintaining protein integrity is paramount. Many labs choose this buffer when preparing samples for activities such as mass spectrometry or Western blotting, where purity and functionality are critical. -
Прашање:
What is the storage requirement for Chaps Lysis Buffer?
Одговор: Chaps Lysis Buffer should be stored in a cool, dry place, typically at 4 °C for short-term storage and -20 °C for long-term use. It's important to protect it from light and avoid repeated freeze-thaw cycles, as these can degrade the buffer’s effectiveness. Researchers often aliquot the buffer into smaller vials to minimize exposure, ensuring reliable results in their experiments and preventing waste of materials in laboratories. -
Прашање:
Does Chaps Lysis Buffer contain any hazardous substances?
Одговор: Chaps Lysis Buffer is generally considered safe to use; however, like all biochemical reagents, it should be handled with care. Protective gloves and eyewear are recommended to avoid any skin or eye contact. Additionally, always refer to the safety data sheet (SDS) for specific handling instructions related to the components in the buffer. Proper lab safety protocols are essential to prevent any potential mishaps during laboratory work. -
Прашање:
How does Chaps compare with other lysis buffers?
Одговор: Chaps Lysis Buffer is often compared to other lysis buffers like Triton X-100 and NP-40, each of which has specific applications. Chaps is milder and is preferred when the preservation of protein activity is critical, while other buffers may be more effective for disrupting various types of cells. This comparative nature allows researchers to choose based on their specific application needs, making it an essential component in many molecular biology protocols. -
Прашање:
Can I use Chaps Lysis Buffer in Western blot experiments?
Одговор: Absolutely! Chaps Lysis Buffer is commonly utilized in Western blotting due to its ability to extract proteins while maintaining their structure and function. The preservation of protein activity is vital in this technique, ensuring accurate results during detection and analysis. Many researchers prefer Chaps for Western blots when targeting membrane proteins, as it provides a more favorable environment for their solubility. -
Прашање:
Where can I buy Chaps Lysis Buffer - Each 50ml?
Одговор: You can purchase Chaps Lysis Buffer - Each 50ml from Ubuy in North Macedonia. Ubuy is a reliable platform that provides access to high-quality laboratory reagents and supplies, making it easy for researchers to find the products they need for their experiments. By choosing Ubuy, you can rest assured of good product quality and swift service tailored to laboratory needs.
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Добрите
- Effective cell lysis
- User-friendly formula
- Compatible with various samples
- Reliable results
- Well-reviewed by researchers
Конс
- Bottle size may be small for some users.
Product Price History
Важни информации
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MKD 5589
Нарачајте сега и ќе се испорача околу Петок, Јули 03
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Ubuy works hard to protect your security and privacy. Our advanced payment security system ensures confidentiality by encrypting your information during transmission using AES (Advanced Encryption Standards) and SSL (Secure Socket Layer) protocols. Your payment details are 100% secure as we do not share your payment details with third party sellers.
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Карактеристики и придобивки
- ISO certified facility ensures top quality and safety.
- Ideal for cell lysis and protein extraction.
- Complies with stringent Good Manufacturing Practices.
- Produced with rigorous quality control under clean room conditions.
- Contains critical components for effective lysis.
- Lab use only, not intended for clinical use.